6ucu | pdb_00006ucu

Cryo-EM structure of the mitochondrial TOM complex from yeast (dimer)

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Structural highlights

6ucu is a 10 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	LMT
Gene:	TOM40, ISP42, MOM38, YMR203W, YM8325.04 (Baker's yeast), TOM22, MAS17, MAS22, MOM22, YNL131W, N1217, N1862 (Baker's yeast), TOM5, MOM8A, YPR133W-A (Baker's yeast), TOM6, ISP6, YOR045W (Baker's yeast), TOM7, MOM7, YNL070W, N2378 (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TOM6_YEAST] Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. TOM6 is involved in assembly and stability of the TOM complex.^[1] [TOM40_YEAST] Channel-forming protein essential for import of protein precursors into mitochondria.^[2] [TOM5_YEAST] Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. TOM5 is involved in insertion of preproteins into the TOM40 translocation pore.^[3] [TOM22_YEAST] Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM70 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Docks TOM20 and TOM70 for interaction with the general TOM40 import pore (GIP) complex. May regulate the TOM machinery organization, stability and channel gating.^[4] ^[5] ^[6] [TOM7_YEAST] Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. TOM7 is involved in assembly and stability of the TOM complex.^[7]

Publication Abstract from PubMed

Nearly all mitochondrial proteins are encoded by the nuclear genome and imported into mitochondria after synthesis on cytosolic ribosomes. These precursor proteins are translocated into mitochondria by the TOM complex, a protein-conducting channel in the mitochondrial outer membrane. We have determined high-resolution cryo-EM structures of the core TOM complex from Saccharomyces cerevisiae in dimeric and tetrameric forms. Dimeric TOM consists of two copies each of five proteins arranged in two-fold symmetry: pore-forming beta-barrel protein Tom40 and four auxiliary alpha-helical transmembrane proteins. The pore of each Tom40 has an overall negatively charged inner surface attributed to multiple functionally important acidic patches. The tetrameric complex is essentially a dimer of dimeric TOM, which may be capable of forming higher-order oligomers. Our study reveals the detailed molecular organization of the TOM complex and provides new insights about the mechanism of protein translocation into mitochondria.

Cryo-EM structure of the mitochondrial protein-import channel TOM complex at near-atomic resolution.,Tucker K, Park E Nat Struct Mol Biol. 2019 Nov 18. pii: 10.1038/s41594-019-0339-2. doi:, 10.1038/s41594-019-0339-2. PMID:31740857^[8]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Model K, Meisinger C, Prinz T, Wiedemann N, Truscott KN, Pfanner N, Ryan MT. Multistep assembly of the protein import channel of the mitochondrial outer membrane. Nat Struct Biol. 2001 Apr;8(4):361-70. doi: 10.1038/86253. PMID:11276259 doi:https://dx.doi.org/10.1038/86253
↑ Rapaport D, Neupert W. Biogenesis of Tom40, core component of the TOM complex of mitochondria. J Cell Biol. 1999 Jul 26;146(2):321-31. doi: 10.1083/jcb.146.2.321. PMID:10427088 doi:https://dx.doi.org/10.1083/jcb.146.2.321
↑ Dietmeier K, Honlinger A, Bomer U, Dekker PJ, Eckerskorn C, Lottspeich F, Kubrich M, Pfanner N. Tom5 functionally links mitochondrial preprotein receptors to the general import pore. Nature. 1997 Jul 10;388(6638):195-200. doi: 10.1038/40663. PMID:9217162 doi:https://dx.doi.org/10.1038/40663
↑ van Wilpe S, Ryan MT, Hill K, Maarse AC, Meisinger C, Brix J, Dekker PJ, Moczko M, Wagner R, Meijer M, Guiard B, Honlinger A, Pfanner N. Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase. Nature. 1999 Sep 30;401(6752):485-9. PMID:10519552 doi:https://dx.doi.org/10.1038/46802
↑ Model K, Meisinger C, Prinz T, Wiedemann N, Truscott KN, Pfanner N, Ryan MT. Multistep assembly of the protein import channel of the mitochondrial outer membrane. Nat Struct Biol. 2001 Apr;8(4):361-70. doi: 10.1038/86253. PMID:11276259 doi:https://dx.doi.org/10.1038/86253
↑ Honlinger A, Kubrich M, Moczko M, Gartner F, Mallet L, Bussereau F, Eckerskorn C, Lottspeich F, Dietmeier K, Jacquet M, et al.. The mitochondrial receptor complex: Mom22 is essential for cell viability and directly interacts with preproteins. Mol Cell Biol. 1995 Jun;15(6):3382-9. PMID:7760834
↑ Model K, Meisinger C, Prinz T, Wiedemann N, Truscott KN, Pfanner N, Ryan MT. Multistep assembly of the protein import channel of the mitochondrial outer membrane. Nat Struct Biol. 2001 Apr;8(4):361-70. doi: 10.1038/86253. PMID:11276259 doi:https://dx.doi.org/10.1038/86253
↑ Tucker K, Park E. Cryo-EM structure of the mitochondrial protein-import channel TOM complex at near-atomic resolution. Nat Struct Mol Biol. 2019 Nov 18. pii: 10.1038/s41594-019-0339-2. doi:, 10.1038/s41594-019-0339-2. PMID:31740857 doi:https://dx.doi.org/10.1038/s41594-019-0339-2

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OCA

[1] Model K, Meisinger C, Prinz T, Wiedemann N, Truscott KN, Pfanner N, Ryan MT. Multistep assembly of the protein import channel of the mitochondrial outer membrane. Nat Struct Biol. 2001 Apr;8(4):361-70. doi: 10.1038/86253. PMID:11276259 doi:https://dx.doi.org/10.1038/86253

[2] Rapaport D, Neupert W. Biogenesis of Tom40, core component of the TOM complex of mitochondria. J Cell Biol. 1999 Jul 26;146(2):321-31. doi: 10.1083/jcb.146.2.321. PMID:10427088 doi:https://dx.doi.org/10.1083/jcb.146.2.321

[3] Dietmeier K, Honlinger A, Bomer U, Dekker PJ, Eckerskorn C, Lottspeich F, Kubrich M, Pfanner N. Tom5 functionally links mitochondrial preprotein receptors to the general import pore. Nature. 1997 Jul 10;388(6638):195-200. doi: 10.1038/40663. PMID:9217162 doi:https://dx.doi.org/10.1038/40663

[4] van Wilpe S, Ryan MT, Hill K, Maarse AC, Meisinger C, Brix J, Dekker PJ, Moczko M, Wagner R, Meijer M, Guiard B, Honlinger A, Pfanner N. Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase. Nature. 1999 Sep 30;401(6752):485-9. PMID:10519552 doi:https://dx.doi.org/10.1038/46802

[5] Model K, Meisinger C, Prinz T, Wiedemann N, Truscott KN, Pfanner N, Ryan MT. Multistep assembly of the protein import channel of the mitochondrial outer membrane. Nat Struct Biol. 2001 Apr;8(4):361-70. doi: 10.1038/86253. PMID:11276259 doi:https://dx.doi.org/10.1038/86253

[6] Honlinger A, Kubrich M, Moczko M, Gartner F, Mallet L, Bussereau F, Eckerskorn C, Lottspeich F, Dietmeier K, Jacquet M, et al.. The mitochondrial receptor complex: Mom22 is essential for cell viability and directly interacts with preproteins. Mol Cell Biol. 1995 Jun;15(6):3382-9. PMID:7760834

[7] Model K, Meisinger C, Prinz T, Wiedemann N, Truscott KN, Pfanner N, Ryan MT. Multistep assembly of the protein import channel of the mitochondrial outer membrane. Nat Struct Biol. 2001 Apr;8(4):361-70. doi: 10.1038/86253. PMID:11276259 doi:https://dx.doi.org/10.1038/86253

[8] Tucker K, Park E. Cryo-EM structure of the mitochondrial protein-import channel TOM complex at near-atomic resolution. Nat Struct Mol Biol. 2019 Nov 18. pii: 10.1038/s41594-019-0339-2. doi:, 10.1038/s41594-019-0339-2. PMID:31740857 doi:https://dx.doi.org/10.1038/s41594-019-0339-2

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6ucu | pdb_00006ucu

Cryo-EM structure of the mitochondrial TOM complex from yeast (dimer)

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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