5c8b | pdb_00005c8b

Structural insights into the redesign of a sucrose phosphorylase by induced loop repositioning

spinqualitylabelsall models 5c8b, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5c8b is a 1 chain structure with sequence from As 1.2190. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BGC Gene: sucP (AS 1.2190) Activity: Transferase, with EC number 2.4.1.7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Sucrose phosphorylases have been applied in the enzymatic production of glycosylated compounds for decades. However, several desirable acceptors, such as flavonoids or stilbenoids, that exhibit diverse antimicrobial, anticarcinogenic or antioxidant properties, remain poor substrates. The Q345F exchange in sucrose phosphorylase from Bifidobacterium adolescentis allows efficient glucosylation of resveratrol, (+)-catechin and (-)-epicatechin in yields of up to 97 % whereas the wild-type enzyme favours sucrose hydrolysis. Three previously undescribed products are made available. The crystal structure of the variant reveals a widened access channel with a hydrophobic aromatic surface that is likely to contribute to the improved activity towards aromatic acceptors. The generation of this channel can be explained in terms of a cascade of structural changes arising from the Q345F exchange. The observed mechanisms are likely to be relevant for the design of other tailor-made enzymes. Redesign of the Active Site of Sucrose Phosphorylase through a Clash-Induced Cascade of Loop Shifts.,Kraus M, Grimm C, Seibel J Chembiochem. 2016 Jan 1;17(1):33-6. doi: 10.1002/cbic.201500514. Epub 2015 Dec 2. PMID:26527586 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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