6dru | pdb_00006dru

Xylosidase from Aspergillus niger

spinqualitylabelsall models 6dru, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6dru is a 2 chain structure with sequence from A. niger. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BGC, BMA, GAL, GOL, MAN, NAG, XYS Gene: ATCC64974_10330, CAN33_17365 (A. niger) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Glycoside hydrolase family 31 (GH31) enzymes show both highly conserved folds and catalytic residues. Yet different members of GH31 show very different substrate specificities, and it is not obvious how these specificities arise from the protein sequences. The fungal alpha-xylosidase, AxlA, was originally isolated from a commercial enzyme mixture secreted by Aspergillus niger and was reported to have potential as a catalytic component in biomass deconstruction in the biofuel industry. We report here the crystal structure of AxlA in complex with its catalytic product, a hydrolyzed xyloglucan oligosaccharide. On the basis of our new structure, we provide the structural basis for AxlA's role in xyloglucan utilization and, more importantly, a new procedure to predict and differentiate C5 vs C6 sugar specific activities based on protein sequences of the functionally diverse GH31 family enzymes. Crystal Structure of alpha-Xylosidase from Aspergillus niger in Complex with a Hydrolyzed Xyloglucan Product and New Insights in Accurately Predicting Substrate Specificities of GH31 Family Glycosidases.,Cao H, Walton JD, Brumm P, Phillips GN Jr ACS Sustain Chem Eng. 2020 Feb 17;8(6):2540-2547. doi:, 10.1021/acssuschemeng.9b07073. Epub 2020 Jan 23. PMID:32161692 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Xylosidase

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