2g5b | pdb_00002g5b

Crystal Structure of the anti-Bax monoclonal antibody 6A7 and a Bax peptide.

spinqualitylabelsall models 2g5b, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2g5b is a 12 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BMA, MAN, NAG, NDG, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The Bcl-2 family member Bax plays a critical role in apoptosis. In healthy resting cells, Bax resides in the cytoplasm and loosely attached to the mitochondrial membrane. Apoptotic stimuli induce Bax activation, which is characterized by translocation and multimerization on the mitochondrial membrane surface resulting in exposure of an amino terminal epitope recognized by the monoclonal antibody 6A7. To understand the structural changes that occur during Bax activation, we determined the crystal structure of a Bax peptide bound to the 6A7 Fab fragment to a resolution of 2.3 A. The structure reveals the conformation of the 6A7 peptide epitope on Bax in the activated form and elucidates the extensive structural changes that Bax must undergo during the conversion from its native to its activated conformation. Elucidation of some Bax conformational changes through crystallization of an antibody-peptide complex.,Peyerl FW, Dai S, Murphy GA, Crawford F, White J, Marrack P, Kappler JW Cell Death Differ. 2007 Mar;14(3):447-52. Epub 2006 Sep 1. PMID:16946732 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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