5d5y | pdb_00005d5y

Structure of Chaetomium thermophilum Skn7 coiled-coil domain, crystal form I

spinqualitylabelsall models 5d5y, resolution 1.03Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5d5y is a 2 chain structure with sequence from Cbs 144.50. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: CTHT_0048700 (CBS 144.50) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Heat-shock transcription factor 1 (HSF1) has a central role in mediating the protective response to protein conformational stresses in eukaryotes. HSF1 consists of an N-terminal DNA-binding domain (DBD), a coiled-coil oligomerization domain, a regulatory domain and a transactivation domain. Upon stress, HSF1 trimerizes via its coiled-coil domain and binds to the promoters of heat shock protein-encoding genes. Here, we present cocrystal structures of the human HSF1 DBD in complex with cognate DNA. A comparative analysis of the HSF1 paralog Skn7 from Chaetomium thermophilum showed that single amino acid changes in the DBD can switch DNA binding specificity, thus revealing the structural basis for the interaction of HSF1 with cognate DNA. We used a crystal structure of the coiled-coil domain of C. thermophilum Skn7 to develop a model of the active human HSF1 trimer in which HSF1 embraces the heat-shock-element DNA. Structure of human heat-shock transcription factor 1 in complex with DNA.,Neudegger T, Verghese J, Hayer-Hartl M, Hartl FU, Bracher A Nat Struct Mol Biol. 2016 Jan 4. doi: 10.1038/nsmb.3149. PMID:26727489 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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