3j0b | pdb_00003j0b
cryo-EM reconstruction of West Nile virus
Structural highlights
Function[Q9Q6P4_WNV] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS011998_004_099774] Publication Abstract from PubMedCoordinated interplay between membrane proteins and the lipid bilayer is required for such processes as transporter function and the entrance of enveloped viruses into host cells. In this study, three-dimensional cryo-electron microscopy density maps of mature and immature flaviviruses were analyzed to assess the curvature of the membrane leaflets and its relation to membrane-bound viral glycoproteins. The overall morphology of the viral membrane is determined by icosahedral scaffolding composed of envelope (E) and membrane (M) proteins through interaction of the proteins' stem-anchor regions with the membrane. In localized regions, small membrane regions exhibit convex, concave, flat or saddle-shaped surfaces that are constrained by the specific protein organization within each membrane leaflet. These results suggest that the organization of membrane proteins in small enveloped viruses mediate the formation of membrane curvature. Membrane curvature in flaviviruses.,Zhang W, Kaufmann B, Chipman PR, Kuhn RJ, Rossmann MG J Struct Biol. 2013 Apr 18. pii: S1047-8477(13)00100-7. doi:, 10.1016/j.jsb.2013.04.005. PMID:23602814[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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