3j9d | pdb_00003j9d

Atomic structure of a non-enveloped virus reveals pH sensors for a coordinated process of cell entry

Loading... Status: waiting for Mol* initialization Structural highlights 3j9d is a 1 chain structure with sequence from Bluetongue virus 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ZN Related: 3j9e Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Viruses sense environmental cues such as pH to engage in membrane interactions for cell entry during infection, but how nonenveloped viruses sense pH is largely undefined. Here, we report both high- and low-pH structures of bluetongue virus (BTV), which enters cells via a two-stage endosomal process. The receptor-binding protein VP2 possesses a zinc finger that may function to maintain VP2 in a metastable state and a conserved His866, which senses early-endosomal pH. The membrane-penetration protein VP5 has three domains: dagger, unfurling and anchoring. Notably, the beta-meander motif of the anchoring domain contains a histidine cluster that can sense late-endosomal pH and also possesses four putative membrane-interaction elements. Exposing BTV to low pH detaches VP2 and dramatically refolds the dagger and unfurling domains of VP5. Our biochemical and structure-guided-mutagenesis studies support these coordinated pH-sensing mechanisms. Atomic model of a nonenveloped virus reveals pH sensors for a coordinated process of cell entry.,Zhang X, Patel A, Celma CC, Yu X, Roy P, Zhou ZH Nat Struct Mol Biol. 2015 Dec 7. doi: 10.1038/nsmb.3134. PMID:26641711 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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