5mxn | pdb_00005mxn

Atomic model of the VipA/VipB/Hcp, the type six secretion system non-contractile sheath-tube of Vibrio cholerae from cryo-EM

Loading... Status: waiting for Mol* initialization Structural highlights 5mxn is a 18 chain structure with sequence from "bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: hcp, hcpA, BFX32_16515, DA89_871, DN30_6, EN12_06865, EN12_14165 ("Bacillo virgola del Koch" Trevisan 1884), BFX10_13915, BFX32_16935, DA89_2095, DN30_1329, EN12_14575, ERS013193_00899 ("Bacillo virgola del Koch" Trevisan 1884), BFX10_13910, BFX32_16930, DA89_2094, DN30_1328, EN12_14570 ("Bacillo virgola del Koch" Trevisan 1884) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The bacterial type VI secretion system (T6SS) uses contraction of a long sheath to quickly thrust a tube with associated effectors across membranes of eukaryotic and bacterial cells 1-5 . Only limited structural information is available about the inherently unstable precontraction state of the T6SS. Here, we obtain a 3.7 A resolution structure of a non-contractile sheath-tube complex using cryo-electron microscopy and show that it resembles the extended T6SS inside Vibrio cholerae cells. We build a pseudo-atomic model of the complete sheath-tube assembly, which provides a mechanistic understanding of coupling sheath contraction with pushing and rotating the inner tube for efficient target membrane penetration. Our data further show that sheath contraction exposes a buried recognition domain to specifically trigger the disassembly and recycling of the T6SS sheath by the cognate ATP-dependent unfoldase ClpV.The structure of the extended sheath-tube complex of the type VI secretion system from Vibrio cholerae elucidates the molecular mechanisms by which conformational changes in the sheath enable the inner tube to penetrate target cells.The structure of the extended sheath-tube complex of the type VI secretion system from Vibrio cholerae elucidates the molecular mechanisms by which conformational changes in the sheath enable the inner tube to penetrate target cells. Cryo-EM structure of the extended type VI secretion system sheath-tube complex.,Wang J, Brackmann M, Castano-Diez D, Kudryashev M, Goldie KN, Maier T, Stahlberg H, Basler M Nat Microbiol. 2017 Sep 25. doi: 10.1038/s41564-017-0020-7. PMID:28947741 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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