5u0a | pdb_00005u0a

CRISPR RNA-guided surveillance complex

Loading... Status: waiting for Mol* initialization Structural highlights 5u0a is a 14 chain structure with sequence from Thefy. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 5u07 Gene: Tfu_1588 (THEFY), Tfu_1592 (THEFY), Tfu_1590 (THEFY), Tfu_1591 (THEFY), Tfu_1589 (THEFY) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Type I CRISPR systems feature a sequential dsDNA target searching and degradation process, by crRNA-displaying Cascade and nuclease-helicase fusion enzyme Cas3, respectively. Here we present two cryo-EM snapshots of the Thermobifida fusca type I-E Cascade: (1) unwinding 11 bp of dsDNA at the seed-sequence region to scout for sequence complementarity, and (2) further unwinding of the entire protospacer to form a full R-loop. These structures provide the much-needed temporal and spatial resolution to resolve key mechanistic steps leading to Cas3 recruitment. In the early steps, PAM recognition causes severe DNA bending, leading to spontaneous DNA unwinding to form a seed-bubble. The full R-loop formation triggers conformational changes in Cascade, licensing Cas3 to bind. The same process also generates a bulge in the non-target DNA strand, enabling its handover to Cas3 for cleavage. The combination of both negative and positive checkpoints ensures stringent yet efficient target degradation in type I CRISPR-Cas systems. Structure Basis for Directional R-loop Formation and Substrate Handover Mechanisms in Type I CRISPR-Cas System.,Xiao Y, Luo M, Hayes RP, Kim J, Ng S, Ding F, Liao M, Ke A Cell. 2017 Jun 29;170(1):48-60.e11. doi: 10.1016/j.cell.2017.06.012. PMID:28666122 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also CRISPR type I-E (Cascade)

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