6e9e | pdb_00006e9e

EsCas13d-crRNA binary complex

Loading... Status: waiting for Mol* initialization Structural highlights 6e9e is a 2 chain structure with sequence from Eubacterium siraeum dsm 15702. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG Gene: EUBSIR_02687 (Eubacterium siraeum DSM 15702) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and their signature Cas13d ribonuclease employs guide RNAs to cleave matching target RNAs. To understand the molecular basis for Cas13d function and explain its compact molecular architecture, we resolved cryoelectron microscopy structures of Cas13d-guide RNA binary complex and Cas13d-guide-target RNA ternary complex to 3.4 and 3.3 A resolution, respectively. Furthermore, a 6.5 A reconstruction of apo Cas13d combined with hydrogen-deuterium exchange revealed conformational dynamics that have implications for RNA scanning. These structures, together with biochemical and cellular characterization, provide insights into its RNA-guided, RNA-targeting mechanism and delineate a blueprint for the rational design of improved transcriptome engineering technologies. Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d.,Zhang C, Konermann S, Brideau NJ, Lotfy P, Wu X, Novick SJ, Strutzenberg T, Griffin PR, Hsu PD, Lyumkis D Cell. 2018 Sep 20;175(1):212-223.e17. doi: 10.1016/j.cell.2018.09.001. PMID:30241607 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also CRISPR type VI

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