6j7v | pdb_00006j7v

Structure of HRPV6 VP5 fitted in the cryoEM density of the spike

Loading... Status: waiting for Mol* initialization Structural highlights 6j7v is a 1 chain structure with sequence from Halorubrum pleomorphic virus 6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 6q6l Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Lipid membrane fusion is an essential function in many biological processes. Detailed mechanisms of membrane fusion and the protein structures involved have been mainly studied in eukaryotic systems, whereas very little is known about membrane fusion in prokaryotes. Haloarchaeal pleomorphic viruses (HRPVs) have a membrane envelope decorated with spikes that are presumed to be responsible for host attachment and membrane fusion. Here we determine atomic structures of the ectodomains of the 57-kDa spike protein VP5 from two related HRPVs revealing a previously unreported V-shaped fold. By Volta phase plate cryo-electron tomography we show that VP5 is monomeric on the viral surface, and we establish the orientation of the molecules with respect to the viral membrane. We also show that the viral membrane fuses with the host cytoplasmic membrane in a process mediated by VP5. This sheds light on protein structures involved in prokaryotic membrane fusion. The structure of a prokaryotic viral envelope protein expands the landscape of membrane fusion proteins.,El Omari K, Li S, Kotecha A, Walter TS, Bignon EA, Harlos K, Somerharju P, De Haas F, Clare DK, Molin M, Hurtado F, Li M, Grimes JM, Bamford DH, Tischler ND, Huiskonen JT, Stuart DI, Roine E Nat Commun. 2019 Feb 19;10(1):846. doi: 10.1038/s41467-019-08728-7. PMID:30783086 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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