6klo | pdb_00006klo

Complex structure of Iota toxin enzymatic component (Ia) and binding component (Ib) pore with short stem

Loading... Status: waiting for Mol* initialization Structural highlights 6klo is a 8 chain structure with sequence from "bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The iota toxin produced by Clostridium perfringens type E is a binary toxin comprising two independent polypeptides: Ia, an ADP-ribosyltransferase, and Ib, which is involved in cell binding and translocation of Ia across the cell membrane. Here we report cryo-EM structures of the translocation channel Ib-pore and its complex with Ia. The high-resolution Ib-pore structure demonstrates a similar structural framework to that of the catalytic varphi-clamp of the anthrax protective antigen pore. However, the Ia-bound Ib-pore structure shows a unique binding mode of Ia: one Ia binds to the Ib-pore, and the Ia amino-terminal domain forms multiple weak interactions with two additional Ib-pore constriction sites. Furthermore, Ib-binding induces tilting and partial unfolding of the Ia N-terminal alpha-helix, permitting its extension to the varphi-clamp gate. This new mechanism of N-terminal unfolding is crucial for protein translocation. Cryo-EM structures reveal translocational unfolding in the clostridial binary iota toxin complex.,Yamada T, Yoshida T, Kawamoto A, Mitsuoka K, Iwasaki K, Tsuge H Nat Struct Mol Biol. 2020 Mar;27(3):288-296. doi: 10.1038/s41594-020-0388-6. Epub, 2020 Mar 2. PMID:32123390 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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