5dus | pdb_00005dus

Crystal structure of MERS-CoV macro domain in complex with ADP-ribose

spinqualitylabelsall models 5dus, resolution 1.43Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5dus is a 1 chain structure with sequence from Mers. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: APR, GOL, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The newly emerging Middle East respiratory syndrome coronavirus (MERS-CoV) encodes the conserved macro domain within non-structural protein 3. However, the precise biochemical function and structure of the macro domain is unclear. Using differential scanning fluorimetry and isothermal titration calorimetry, we characterized the MERS-CoV macro domain as a more efficient adenosine diphosphate (ADP)-ribose binding module than macro domains from other CoVs. Furthermore, the crystal structure of the MERS-CoV macro domain was determined at 1.43-A resolution in complex with ADP-ribose. Comparison of macro domains from MERS-CoV and other human CoVs revealed structural differences in the alpha1 helix alters how the conserved Asp-20 interacts with ADP-ribose and may explain the efficient binding of the MERS-CoV macro domain to ADP-ribose. This study provides structural and biophysical bases to further evaluate the role of the MERS-CoV macro domain in the host response via ADP-ribose binding but also as a potential target for drug design. Macro Domain from Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Is an Efficient ADP-ribose Binding Module: CRYSTAL STRUCTURE AND BIOCHEMICAL STUDIES.,Cho CC, Lin MH, Chuang CY, Hsu CH J Biol Chem. 2016 Mar 4;291(10):4894-902. doi: 10.1074/jbc.M115.700542. Epub 2016, Jan 5. PMID:26740631 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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