5e41 | pdb_00005e41

Crystal structure of the large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with 5-(N-(10-hydroxydecanoyl)-aminopentenyl)-2'-deoxyuridine-triphosphate

spinqualitylabelsall models 5e41, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5e41 is a 3 chain structure with sequence from Atcc 25104. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: AUL, GOL, MG NonStd Res: DOC Gene: polA, pol1 (ATCC 25104) Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Efficient incorporation of modified nucleotides by DNA polymerases is essential for many cutting-edge biomolecular technologies. The present study compares the acceptance of either alkene- or alkyne-modified nucleotides by KlenTaq DNA polymerase and provides structural insights into how 7-deaza-adenosine and thymidine with attached alkene-modifications are incorporated into the growing DNA strand. Thereby, we identified modified nucleotides, which prove to be superior substrates for KlenTaq DNA polymerase compared to their natural analogues. The knowledge can be used to guide future design of functionalized nucleotide building blocks. Structural basis for the KlenTaq DNA Polymerase catalyzed Incorporation of Alkene- versus Alkyne-modified Nucleotides.,Marx A, Hottin A, Betz K, Diederichs K Chemistry. 2016 Nov 30. doi: 10.1002/chem.201604515. PMID:27901305 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also DNA polymerase 3D structures

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