5esx | pdb_00005esx

Crystal structure of hypoxanthine-guanine phosphoribosyltransferase complexed with GMP from Legionella pneumophila

spinqualitylabelsall models 5esx, resolution 2.71Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5esx is a 2 chain structure with sequence from Legionella pneumophila subsp. pneumophila 570-co-h. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 5GP Related: 5esw Gene: lp12_1457 (Legionella pneumophila subsp. pneumophila 570-CO-H) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) (EC 2.4.2.8) reversibly catalyzes the transfer of the 5-phophoribosyl group from 5-phosphoribosyl-alpha-1-pyrophosphate (PRPP) to hypoxanthine or guanine to form inosine monophosphate (IMP) or guanosine monophosphate (GMP) in the purine salvage pathway. To investigate the catalytic mechanism of this enzyme in the intracellular pathogen Legionella pneumophila, we determined the crystal structures of the L. pneumophila HGPRT (LpHGPRT) both in its apo-form and in complex with GMP. The structures reveal that LpHGPRT comprises a core domain and a hood domain which are packed together to create a cavity for GMP-binding and the enzymatic catalysis. The binding of GMP induces conformational changes of the stable loop II. This new binding site is closely related to the Gout arthritis-linked human HGPRT mutation site (Ser103Arg). Finally, these structures of LpHGPRT provide insights into the catalytic mechanism of HGPRT. Crystal structures of Apo and GMP bound hypoxanthine-guanine phosphoribosyltransferase from Legionella pneumophila and the implications in gouty arthritis.,Zhang N, Gong X, Lu M, Chen X, Qin X, Ge H J Struct Biol. 2016 Jun;194(3):311-6. doi: 10.1016/j.jsb.2016.03.007. Epub 2016, Mar 8. PMID:26968365 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Phosphoribosyltransferase 3D structures

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