5gl6 | pdb_00005gl6
Msmeg rimP
Structural highlights
Function[RIMP_MYCS2] Required for maturation of 30S ribosomal subunits.[HAMAP-Rule:MF_01077] Publication Abstract from PubMedThe ribosomal maturation factor P (RimP) is a highly conserved protein in bacteria and has been shown to be important in ribosomal assembly in Escherichia coli Because of its central importance in bacterial metabolism, RimP represents a good potential target for drug design to combat human pathogens such as Mycobacterium tuberculosis However, to date, the only RimP structure available is the NMR structure of the ortholog in another bacterial pathogen, Streptococcus pneumoniae Here, we report a 2.2 A resolution crystal structure of MSMEG_2624, the RimP ortholog in the close M. tuberculosis relative Mycobacterium smegmatis, and using in vitro binding assays, we show that MSMEG_2624 interacts with the small ribosomal protein S12, also known as RpsL. Further analyses revealed that the conserved residues in the linker region between the N- and C-terminal domains of MSMEG_2624 are essential for binding to RpsL. However, neither of the two domains alone was sufficient to form strong interactions with RpsL. More importantly, the linker region was essential for in vivo ribosomal biogenesis. Our study provides critical mechanistic insights into the role of RimP in ribosome biogenesis. We anticipate that the MSMEG_2624 crystal structure has the potential to be used for drug design to manage M. tuberculosis infections. The ribosomal maturation factor P from Mycobacterium smegmatis facilitates the ribosomal biogenesis by binding to the small ribosomal protein S12.,Chu T, Weng X, Law COK, Kong HK, Lau J, Li S, Pham HQ, Wang R, Zhang L, Kao RYT, Lau KF, Ngo JCK, Lau TCK J Biol Chem. 2019 Jan 4;294(1):372-378. doi: 10.1074/jbc.RA118.002298. Epub 2018 , Nov 8. PMID:30409901[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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