6w98 | pdb_00006w98

Single-Particle Cryo-EM Structure of Arabinofuranosyltransferase AftD from Mycobacteria

spinqualitylabelsall models 6w98, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6w98 is a 2 chain structure with sequence from [1] and Escherichia coli (strain k12). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 6OU, CA, PNS Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [ACP_ECOLI] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217] Publication Abstract from PubMed Mycobacterium tuberculosis causes tuberculosis, a disease that kills over 1 million people each year. Its cell envelope is a common antibiotic target and has a unique structure due, in part, to two lipidated polysaccharides-arabinogalactan and lipoarabinomannan. Arabinofuranosyltransferase D (AftD) is an essential enzyme involved in assembling these glycolipids. We present the 2.9-A resolution structure of M. abscessus AftD, determined by single-particle cryo-electron microscopy. AftD has a conserved GT-C glycosyltransferase fold and three carbohydrate-binding modules. Glycan array analysis shows that AftD binds complex arabinose glycans. Additionally, AftD is non-covalently complexed with an acyl carrier protein (ACP). 3.4- and 3.5-A structures of a mutant with impaired ACP binding reveal a conformational change, suggesting that ACP may regulate AftD function. Mutagenesis experiments using a conditional knockout constructed in M. smegmatis confirm the essentiality of the putative active site and the ACP binding for AftD function. Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria.,Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosario AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalao MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F Mol Cell. 2020 May 5. pii: S1097-2765(20)30256-2. doi:, 10.1016/j.molcel.2020.04.014. PMID:32386575 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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