5h20 | pdb_00005h20

X-ray structure of PadR-like Transcription factor from bacteroid fragilis

spinqualitylabelsall models 5h20, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5h20 is a 1 chain structure with sequence from Bacfn. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GLC, IPA, PO4 Gene: BF9343_2549 (BACFN) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed A phenolic acid decarboxylase (padC) regulator, PadR and its homologs proteins belong to the PadR family. Despite the growing numbers of the PadR family members and their various roles in bacteria, such as detoxifications, drug transports and circadian rhythms, biochemical and biophysical studies of the PadR family are very limited. Thus, a ligand-induced regulatory mechanism of the PadR family transcription factors remains to be elucidated. Here, we report a crystal structure of a Bacteroides fragilis PadR-like protein, BF2549 and revealed its interaction with putative operator DNA and ligand molecules. Comparative structural and primary sequence analyses provide a PadR-specific motif that is conserved in the PadR family but deviated from the MarR family. Furthermore, putative ligand binding sites are observed in the BF2549 structure. Finally, a homology-based structure model of BF2549 and 29-mer dsDNA propose regulatory mechanisms of the PadR family in transcriptional derepression. Structural and functional analysis of BF2549, a PadR-like transcription factor from Bacteroides fragilis.,Lee C, Kim MI, Hong M Biochem Biophys Res Commun. 2017 Jan 29;483(1):264-270. doi:, 10.1016/j.bbrc.2016.12.155. Epub 2016 Dec 24. PMID:28027933 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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