Structural highlights
Publication Abstract from PubMedFood processing and refining has dramatically changed the human diet, but little is known about whether this affected the evolution of enzymes in human microbiota. We present evidence that glycoside hydrolase family 70 (GH70) glucansucrases from lactobacilli, synthesizing alpha-glucan-type extracellular polysaccharides from sucrose, likely evolved from GH13 starch-acting alpha-amylases, via GH70 4,6-alpha-glucanotransferases. The crystal structure of a 4,6-alpha-glucanotransferase explains the mode of action and unique product specificity of these enzymes. While the alpha-amylase substrate-binding scaffold is retained, active-site loops adapted to favor transglycosylation over hydrolysis; the structure also gives clues as to how 4,6-alpha-glucanotransferases may have evolved further toward sucrose utilization instead of starch. Further supported by genomic, phylogenetic, and in vivo studies, we propose that dietary changes involving starch (and starch derivatives) and sucrose intake were critical factors during the evolution of 4,6-alpha-GTs and glucansucrases from alpha-amylases, allowing oral bacteria to produce extracellular polymers that contribute to biofilm formation from different substrates. Crystal Structure of 4,6-alpha-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from alpha-Amylases in Oral Bacteria.,Bai Y, Gangoiti J, Dijkstra BW, Dijkhuizen L, Pijning T Structure. 2016 Dec 21. pii: S0969-2126(16)30390-2. doi:, 10.1016/j.str.2016.11.023. PMID:28065507[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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