Structural highlights
Publication Abstract from PubMedPhage G has the largest capsid and genome of any known propagated phage. Many aspects of its structure, assembly, and replication have not been elucidated. Herein, we present the dsDNA-packed and empty phage G capsid at 6.1 and 9A resolution, respectively, using cryo-EM for structure determination and mass spectrometry for protein identification. The major capsid protein, gp27, is identified and found to share the HK97-fold universally conserved in all previously solved dsDNA phages. Trimers of the decoration protein, gp26, sit on the 3-fold axes and are thought to enhance the interactions of the hexameric capsomeres of gp27, for other phages encoding decoration proteins. Phage G's decoration protein is longer than what has been reported in other phages, and we suspect the extra interaction surface area helps stabilize the capsid. We identified several additional capsid proteins, including a candidate for the prohead protease responsible for processing gp27. Furthermore, cryo-EM reveals a range of partially full, condensed DNA densities that appear to have no contact with capsid shell. Three analyses confirm that the phage G host is a Lysinibacillus, and not Bacillus megaterium: identity of host proteins in our mass spectrometry analyses, genome sequence of the phage G host, and host range of phage G. Phage G Structure at 6.1A Resolution, Condensed DNA, and Host Identity Revision to a Lysinibacillus.,Gonzalez B, Monroe L, Li K, Yan R, Wright E, Walter T, Kihara D, Weintraub ST, Thomas JA, Serwer P, Jiang W J Mol Biol. 2020 May 23. pii: S0022-2836(20)30366-1. doi:, 10.1016/j.jmb.2020.05.016. PMID:32454153[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
| ||||||||||||||||
This page was last modified 14:41, 8 July 2020.