6ypf | pdb_00006ypf

NUDIX1 hydrolase from Rosa x hybrida in complex with geranyl pyrophosphate

spinqualitylabelsall models 6ypf, resolution 1.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ypf is a 1 chain structure with sequence from Roshc. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GPP Gene: NUDIX1 (ROSHC) Activity: Geranyl diphosphate phosphohydrolase, with EC number 3.6.1.68 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Roses use a non-canonical pathway involving a Nudix hydrolase, RhNUDX1, to synthesize their monoterpenes, especially geraniol. Here we report the characterization of another expressed NUDX1 gene from the rose cultivar Rosa x wichurana, RwNUDX1-2. In order to study the function of the RwNUDX1-2 protein, we analysed the volatile profiles of an F1 -progeny generated by crossing R. chinensis cv. 'Old Blush' with R. x wichurana. A correlation test of the volatilomes with gene expression data revealed that RwNUDX1-2 is involved in the biosynthesis of a group of sesquiterpenoids: especially E,E-farnesol in addition to other sesquiterpenes. In vitro enzyme assays and heterologous in planta functional characterization of the RwNUDX1-2 gene corroborated this result. A quantitative trait locus (QTL) analysis was performed using the data of E,E-farnesol contents in the progeny and a genetic map constructed based on gene markers. The RwNUDX1-2 gene co-localized with the QTL for E,E-farnesol content, thereby confirming its function in sesquiterpenoid biosynthesis in R. x wichurana. Finally, in order to understand the structural bases for the substrate specificity of rose NUDX proteins, the RhNUDX1 protein was crystallized, and its structure was refined to 1.7 A. By molecular modelling of different rose NUDX1 protein complexes with their respective substrates, a structural basis for substrate discrimination by rose NUDX1 proteins is proposed. Functional diversification in the Nudix hydrolase gene family drives sesquiterpene biosynthesis in Rosa x wichurana.,Sun P, Degut C, Rety S, Caissard JC, Hibrand-Saint Oyant L, Bony A, Paramita SN, Conart C, Magnard JL, Jeauffre J, Abd-El-Haliem AM, Marie-Magdelaine J, Thouroude T, Baltenweck R, Tisne C, Foucher F, Haring M, Hugueney P, Schuurink RC, Baudino S Plant J. 2020 Jul 8. doi: 10.1111/tpj.14916. PMID:32639596 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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