6xpe | pdb_00006xpe

Cryo-EM structure of human ZnT8 WT, in the presence of zinc, determined in outward-facing conformation

spinqualitylabelsall models 6xpe, resolution 4.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6xpe is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ZN Gene: SLC30A8, ZNT8 (HUMAN) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [ZNT8_HUMAN] Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta-cells. Publication Abstract from PubMed ZnT8 is a Zn(2+)/H(+) antiporter that belongs to SLC30 family and plays an essential role in regulating Zn(2+) accumulation in the insulin secretory granules of pancreatic beta cells. Dysfunction of ZnT8 is associated with both type 1 and 2 diabetes. However, the Zn(2+)/H(+) exchange mechanism of ZnT8 remains unclear due to the lack of high-resolution structures. Here, we report the cryo-EM structures of human ZnT8 (HsZnT8) in both outward- and inward-facing conformations. HsZnT8 forms a dimeric structure with four Zn(2+) binding sites within each subunit: a highly conserved primary site in transmembrane domain (TMD) housing the Zn(2+) substrate; an interfacial site between TMD and C-terminal domain (CTD) that modulates the Zn(2+) transport activity of HsZnT8; and two adjacent sites buried in the cytosolic domain and chelated by conserved residues from CTD and the His-Cys-His (HCH) motif from the N-terminal segment of the neighboring subunit. A comparison of the outward- and inward-facing structures reveals that the TMD of each HsZnT8 subunit undergoes a large structural rearrangement, allowing for alternating access to the primary Zn(2+) site during the transport cycle. Collectively, our studies provide the structural insights into the Zn(2+)/H(+) exchange mechanism of HsZnT8. Cryo-EM structures of human ZnT8 in both outward- and inward-facing conformations.,Xue J, Xie T, Zeng W, Jiang Y, Bai XC Elife. 2020 Jul 29;9. pii: 58823. doi: 10.7554/eLife.58823. PMID:32723473 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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