5o69 | pdb_00005o69

The structure of the thermobifida fusca guanidine III riboswitch with agmatine.

spinqualitylabelsall models 5o69, resolution 2.32Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5o69 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: AG2, MG NonStd Res: CBV Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Riboswitches are structural elements found in mRNA molecules that couple small-molecule binding to regulation of gene expression, usually by controlling transcription or translation. We have determined high-resolution crystal structures of the ykkC guanidine III riboswitch from Thermobifida fusca. The riboswitch forms a classic H-type pseudoknot that includes a triple helix that is continuous with a central core of conserved nucleotides. These form a left-handed helical ramp of inter-nucleotide interactions, generating the guanidinium cation binding site. The ligand is hydrogen bonded to the Hoogsteen edges of two guanine bases. The binding pocket has a side opening that can accommodate a small side chain, shown by structures with bound methylguanidine, aminoguanidine, ethylguanidine, and agmatine. Comparison of the new structure with those of the guanidine I and II riboswitches reveals that evolution generated three different structural solutions for guanidine binding and subsequent gene regulation, although with some common elements. Structure of the Guanidine III Riboswitch.,Huang L, Wang J, Wilson TJ, Lilley DMJ Cell Chem Biol. 2017 Sep 28. pii: S2451-9456(17)30319-7. doi:, 10.1016/j.chembiol.2017.08.021. PMID:28988949 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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