6itl | pdb_00006itl

Crystal structure of malate dehydrogenase from Mannheimia succiniciproducens in complex with NAD

spinqualitylabelsall models 6itl, resolution 1.97Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6itl is a 1 chain structure with sequence from Mansm. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, NAD Gene: mdh, MS1266 (MANSM) Activity: Malate dehydrogenase, with EC number 1.1.1.37 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [MDH_MANSM] Catalyzes the reversible oxidation of malate to oxaloacetate. Publication Abstract from PubMed Succinic acid (SA), a dicarboxylic acid of industrial importance, can be efficiently produced by metabolically engineered Mannheimia succiniciproducens. Malate dehydrogenase (MDH) is one of the key enzymes for SA production, but has not been well characterized. Here we report biochemical and structural analyses of various MDHs and development of hyper-SA producing M. succiniciproducens by introducing the best MDH. Corynebacterium glutamicum MDH (CgMDH) shows the highest specific activity and least substrate inhibition, whereas M. succiniciproducens MDH (MsMDH) shows low specific activity at physiological pH and strong uncompetitive inhibition toward oxaloacetate (ki of 67.4 and 588.9 muM for MsMDH and CgMDH, respectively). Structural comparison of the two MDHs reveals a key residue influencing the specific activity and susceptibility to substrate inhibition. A high-inoculum fed-batch fermentation of the final strain expressing cgmdh produces 134.25 g L(-1) of SA with the maximum productivity of 21.3 g L(-1) h(-1), demonstrating the importance of enzyme optimization in strain development. Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase.,Ahn JH, Seo H, Park W, Seok J, Lee JA, Kim WJ, Kim GB, Kim KJ, Lee SY Nat Commun. 2020 Apr 23;11(1):1970. doi: 10.1038/s41467-020-15839-z. PMID:32327663 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Malate Dehydrogenase 3D structures

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