6lep | pdb_00006lep

Crystal structure of thiosulfate transporter YeeE inactive mutant - C91A

spinqualitylabelsall models 6lep, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6lep is a 1 chain structure with sequence from Atcc 700085. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: OLC, THJ Gene: Spith_0734 (ATCC 700085) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed We have demonstrated that a bacterial membrane protein, YeeE, mediates thiosulfate uptake. Thiosulfate is used for cysteine synthesis in bacteria as an inorganic sulfur source in the global biological sulfur cycle. The crystal structure of YeeE at 2.5-A resolution reveals an unprecedented hourglass-like architecture with thiosulfate in the positively charged outer concave side. YeeE is composed of loops and 13 helices including 9 transmembrane alpha helices, most of which show an intramolecular pseudo 222 symmetry. Four characteristic loops are buried toward the center of YeeE and form its central region surrounded by the nine helices. Additional electron density maps and successive molecular dynamics simulations imply that thiosulfate can remain temporally at several positions in the proposed pathway. We propose a plausible mechanism of thiosulfate uptake via three important conserved cysteine residues of the loops along the pathway. Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake.,Tanaka Y, Yoshikaie K, Takeuchi A, Ichikawa M, Mori T, Uchino S, Sugano Y, Hakoshima T, Takagi H, Nonaka G, Tsukazaki T Sci Adv. 2020 Aug 26;6(35):eaba7637. doi: 10.1126/sciadv.aba7637. eCollection, 2020 Aug. PMID:32923628 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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