7cla | pdb_00007cla

Crystal structure of HTH-type transcriptional regulator SkgA from Caulobacter crescentus

spinqualitylabelsall models 7cla, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7cla is a 1 chain structure with sequence from Cauvc. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: skgA, CC_0694 (CAUVC) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [SKGA_CAUVC] Regulates the induction of katG (catalase-peroxidase) in stationary phase. Publication Abstract from PubMed Investigations of bacterial resistance strategies can aid in the development of new antimicrobial drugs as a countermeasure to the increasing worldwide prevalence of bacterial antibiotic resistance. One such strategy involves the TipA class of transcription factors, which constitute minimal autoregulated multidrug resistance (MDR) systems against diverse antibiotics. However, we have insufficient information regarding how antibiotic binding induces transcriptional activation to design molecules that could interfere with this process. To learn more, we determined the crystal structure of SkgA from Caulobacter crescentus as a representative TipA protein. We identified an unexpected spatial orientation and location of the antibiotic binding TipAS effector domain in the apo state. We observed that the alpha6-alpha7 region of the TipAS domain, which is canonically responsible for forming the lid of antibiotic binding cleft to tightly enclose the bound antibiotic, is involved in the dimeric interface and stabilized via interaction with the DNA-binding domain in the apo state. Further structural and biochemical analyses demonstrated that the unliganded TipAS domain sterically hinders promoter DNA binding, but undergoes a remarkable conformational shift upon antibiotic binding to release this autoinhibition via a switch of its alpha6-alpha7 region. Hence, the promoters for MDR genes including tipA and RNA polymerases become available for transcription, enabling efficient antibiotic resistance. These insights into the molecular mechanism of activation of TipA proteins advance our understanding of TipA proteins as well as bacterial MDR systems, and may provide important clues to block bacterial resistance. Antibiotic binding releases autoinhibition of the TipA multidrug-resistance transcriptional regulator.,Jiang X, Zhang L, Teng M, Li X J Biol Chem. 2020 Oct 22. pii: RA120.016295. doi: 10.1074/jbc.RA120.016295. PMID:33093172 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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