7d9m | pdb_00007d9m
grass carp interleukin-2
Structural highlights
Publication Abstract from PubMedInterleukin (IL) -2, a member of the four alpha-helical cytokine family, has broad regulatory roles in mediating vertebrate immune response. In mammals, IL-2 and IL-15 share a common evolutionary origin and possess overlapping but distinct functions. IL-2 and IL-15 bind to distinct private receptors for signaling. However, fish appear to possess a single IL-15Ralpha like gene whilst lack additional gene(s) coding for IL-2Ralpha. Whether the IL-2 and IL-15 interact with the same receptor in fish and how their functions and receptors have evolved are not fully understood. In this study, homologues of IL-2 and IL-2/15Ralpha were sequenced from a teleost species, grass carp (Ctenopharyngodon idella), and the crystal structure of IL-2 was determined. The grass carp IL-2 (termed CiIL-2) displayed a classical cytokine structure consisting of four helical bundles which shares significant similarity with human IL-15. The key amino acids involved in the interface interaction of IL-2/15 and their receptors are well conserved. The CiIL-2 has been shown to bind the IL-2/15Ralpha like homologue with an affinity of 2.45 nM, supporting the notion that fish IL-2 and IL-15 may share a single common private receptor for exerting functions. Syntenic analysis suggests that the IL-2Ralpha of tetrapods has evolved from an IL-15Ralpha like homologue, in which a second sushi domain (D2) in the extracellular region has been duplicated to facilitate the specific interaction with IL-2. The CiIL-2 was predominantly expressed in lymphocyte-rich tissues such as the spleen, kidney and thymus, and could be induced by PHA and IL-21. In vivo challenge with grass carp reovirus and Flavobacterium columnare also resulted in upregulation of CiIL-2 expression. The recombinant CiIL-2 was shown to activate expression of STAT5b, IL-1beta, IL-22 and IFN-gamma, and to promote the proliferation of the primary cell cultures from head kidney leucocytes. Our results shed lights into the co-evolution of IL-2 and its private receptor, and the functional divergence of IL-2 and IL-15 during evolution. Structural insights into the co-evolution of IL-2 and its private receptor in fish.,Wang J, Wang W, Xu J, Jia Z, Liu Q, Zhu X, Xia C, Zou J Dev Comp Immunol. 2020 Oct 14;115:103895. doi: 10.1016/j.dci.2020.103895. PMID:33065202[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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