EPSP synthase

spinqualitylabelsall models Structure of E. coli EPSP synthase complex with shikimate-3-phosphate, the herbicide glyphosate and formic acid (PDB entry 1g6s) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase is a key enzyme for the biosynthesis of aromatic amino acids in plants and many microbes. EPSP synthase catalyzes the addition of phosphoenol pyruvate (PEP) to shikimate-3-phosphate (S3P), generating 5-enolpyruvylshikimate-3-phosphate, which is a precursor for phenylalanine and tyrosine. EPSP synthase is a target for herbicides like Roundup, which contain glyphosate, an inhibitor of EPSP synthase. Herbicide resistant plants contain an glyphosate insensitive version of EPSP synthase derived from Agrobacterium sp strain CP4, so it is called CP4 EPSP synthase. , Structural insights The enzyme has two domains, with the active site found in the interdomain cleft (open conformation). There is a substantial structural change upon substrate binding, resulting in a closed conformation. See animation of this process. Glyphosate (also known as Roundup) occupies the binding site of the second substrate, phosphoenol pyruvate . Interestingly CP4 EPSP synthase still binds glyphosate in the absence of PEP, but a conformational change in glyphosate to accommodate a steric clash with Glu 354 shortens the length of glyphosate, from 7.3 angstroms to 6.67 angstroms, and changes the IC50 by a factor of over 4,000, from 2.5 micromolar to 11 millimolar.

3D structures of EPSP synthase

Updated on 04-November-2020

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• 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase
  • 1eps – EcEPSP – Escherichia coli
    
  • 1p88, 1p89 - EcEPSP N terminal - NMR
    
  • 1rf5 - SpEPSP – Streptococcus pneumoniae
    
  • 2bjb, 2o15 – MtEPSP – Mycobacterium tuberculosis
    
  • 2gg4 – AgEPSP – Agrobacterium
    
  • 3roi, 3tr1, 4gfp – CbEPSP – Coxiella burnetii
    
  • 3ti2 - VcEPSP N terminal – Vibrio cholerae
    
  • 3rmt – EPSP – Bacillus halodurans
    
• EPSP synthase binary complex
  • 1g6t - EcEPSP + S3P
    
  • 1mi4, 2qfq, 2qfs, 3fjx, 3fk0 - EcEPSP (mutant) + S3P
    
  • 1q36 - EcEPSP (mutant) + S3P derivative
    
  • 1x8r, 1x8t - EcEPSP + intermediate
    
  • 2aa9 – EcEPSP + shikimate
    
  • 2pq9 - EcEPSP + intermediate
    
  • 1rf4 - SpEPSP + intermediate
    
  • 2o0b, 2o0d - MtEPSP + S3P
    
  • 2gg6 - AgEPSP + S3P
    
  • 2pqb, 2pqc, 2pqd - AgEPSP + intermediate
    
  • 2o0x - MtEPSP + intermediate
    
  • 2o0z - MtEPSP + EPS
    
  • 4egr - CbEPSP + phosphoenolpyruvate
    
• EPSP synthase ternary complex
  • 1g6s - EcEPSP + S3P + glyphosate
    
  • 2qft, 2qfu, 3fjz, 3fk1 - EcEPSP (mutant) + S3P + glyphosate
    
  • 2aay - EcEPSP + shikimate + glyphosate
    
  • 1rf6 - SpEPSP + S3P + glyphosate
    
  • 2gga - AgEPSP + S3P + glyphosate
    
  • 2ggd - AgEPSP (mutant) + S3P + glyphosate
    
  • 2o0e - MtEPSP + S3P + phosphoenolpyruvate
    
  • 3nvs - VcEPSP + S3P + glyphosate
    
  • 3slh - CbEPSP + S3P + glyphosate
    

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