6yyi | pdb_00006yyi

Crystal structure of beta-D-xylosidase from Dictyoglomus thermophilum bound to beta-D-xylopyranose

spinqualitylabelsall models 6yyi, resolution 2.67Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6yyi is a 2 chain structure with sequence from Dict6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CIT, EDO, NA, SO4, XYP Gene: DICTH_1792 (DICT6) Activity: Xylan 1,4-beta-xylosidase, with EC number 3.2.1.37 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Dictyoglomus thermophilum beta-d-xylosidase DtXyl is attractive as a potential thermostable biocatalyst able to produce biologically active ginsenosides intermediates from beta-(1,2)-D-xylosylated compounds, including Notoginsenoside-R1. DtXyl was expressed as an active N-terminal His-tagged protein, and its crystal structure was solved in presence or absence of d-xylose product. Modelling of notoginsenoside R1 in DtXyl active site led to the identification of several hydrophobic residues interacting in close contact to the substrate hydrophobic core. Unlike other residues involved in substrate binding, these residues are not conserved among GH39 xylosidase family, and their physico-chemical properties can be correlated to the efficient binding and subsequent hydrolysis of Notoginsenoside R1. Crystal structure of Dictyoglomus thermophilum beta-d-xylosidase DtXyl unravels the structural determinants for efficient notoginsenoside R1 hydrolysis.,Bretagne D, Paris A, de Vaumas R, Lafite P, Daniellou R Biochimie. 2020 Nov 23;181:34-41. doi: 10.1016/j.biochi.2020.11.017. PMID:33242495 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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