6wk3 | pdb_00006wk3

Engineered carbene transferase RmaNOD Q52V, putative nitric oxide dioxygenase from Rhodothermus marinus

spinqualitylabelsall models 6wk3, resolution 2.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6wk3 is a 4 chain structure with sequence from Rhom4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ACT, CU, HEM Gene: Rmar_2776 (RHOM4) Activity: Nitric oxide dioxygenase, with EC number 1.14.12.17 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed While biocatalysis is increasingly incorporated into drug development pipelines, it is less commonly used in the early stages of drug discovery. By engineering a protein to produce a chiral motif with a derivatizable functional handle, biocatalysts can be used to help generate diverse building blocks for drug discovery. Here we show the engineering of two variants of Rhodothermus marinus nitric oxide dioxygenase (RmaNOD) to catalyze the formation of cis- and tran- diastereomers of a pinacolboronate-substituted cyclopropane which can be readily derivatized to generate diverse stereopure cyclopropane building blocks. Diversity-Oriented Enzymatic Synthesis of Cyclopropane Building Blocks.,Wittmann BJ, Knight AM, Hofstra JL, Reisman SE, Kan SBJ, Arnold FH ACS Catal. 2020 Jul 2;10(13):7112-7116. doi: 10.1021/acscatal.0c01888. Epub 2020 , Jun 4. PMID:33282460 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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