1sg3 | pdb_00001sg3

Structure of allantoicase

spinqualitylabelsall models 1sg3, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1sg3 is a 2 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: DAL2, ALC1, YIR029W (ATCC 18824) Activity: Allantoicase, with EC number 3.5.3.4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [ALLC_YEAST] Utilization of purines as secondary nitrogen sources, when primary sources are limiting. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Allantoicase (EC 3.5.3.4) catalyzes the conversion of allantoate into ureidoglycolate and urea, one of the final steps in the degradation of purines to urea. The mechanism of most enzymes involved in this pathway, which has been known for a long time, is unknown. In this paper we describe the three-dimensional crystal structure of the yeast allantoicase determined at a resolution of 2.6 A by single anomalous diffraction. This constitutes the first structure for an enzyme of this pathway. The structure reveals a repeated jelly roll beta-sheet motif, also present in proteins of unrelated biochemical function. Allantoicase has a hexameric arrangement in the crystal (dimer of trimers). Analysis of the protein sequence against the structural data reveals the presence of two totally conserved surface patches, one on each jelly roll motif. The hexameric packing concentrates these patches into conserved pockets that probably constitute the active site. Crystal structure of yeast allantoicase reveals a repeated jelly roll motif.,Leulliot N, Quevillon-Cheruel S, Sorel I, Graille M, Meyer P, Liger D, Blondeau K, Janin J, van Tilbeurgh H J Biol Chem. 2004 May 28;279(22):23447-52. Epub 2004 Mar 12. PMID:15020593 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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