6z38 | pdb_00006z38

TodX deltaS2S3 mutant monoaromatic hydrocarbon channel

spinqualitylabelsall models 6z38, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6z38 is a 1 chain structure with sequence from "bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 6z34, 6z37 Gene: todX ("Bacillus fluorescens putidus" Flugge 1886) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed In modern societies, biodegradation of hydrophobic pollutants generated by industry is important for environmental and human health. In Gram-negative bacteria, biodegradation depends on facilitated diffusion of the pollutant substrates into the cell, mediated by specialised outer membrane (OM) channels. Here we show, via a combined experimental and computational approach, that the uptake of monoaromatic hydrocarbons such as toluene in Pseudomonas putida F1 (PpF1) occurs via lateral diffusion through FadL channels. Contrary to classical diffusion channels via which polar substrates move directly into the periplasmic space, PpF1 TodX and CymD direct their hydrophobic substrates into the OM via a lateral opening in the channel wall, bypassing the polar barrier formed by the lipopolysaccharide leaflet on the cell surface. Our study suggests that lateral diffusion of hydrophobic molecules is the modus operandi of all FadL channels, with potential implications for diverse areas such as biodegradation, quorum sensing and gut biology. Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusion.,Somboon K, Doble A, Bulmer D, Basle A, Khalid S, van den Berg B Nat Commun. 2020 Dec 10;11(1):6331. doi: 10.1038/s41467-020-20126-y. PMID:33303757 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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