Sandbox Reserved 1654

This Sandbox is Reserved from 26/11/2020, through 26/11/2021 for use in the course "Structural Biology" taught by Bruno Kieffer at the University of Strasbourg, ESBS. This reservation includes Sandbox Reserved 1643 through Sandbox Reserved 1664.

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Cytplasmic Polyadenylation Element-binding Protein (CPEB)

You may include any references to papers as in: the use of JSmol in Proteopedia or to the article describing Jmol to the rescue.

Structure

All CPEB proteins have a similar structure :

• A N-terminal region which is a regulatory region with phosphorylation and dephosphorylation sites. This region is variable in length and composition.
• A C-terminal region, composed of 2 recognition patterns (RRMs which allow a good positioning of RNA, a high fidelity and are essential for the CPE specific recognition) and 2 zinc finger patterns (containing two zinc binding sites and a specific RNA-binding protein sequence which play a role in affinity but not in specificity).
  • Zinc finger patterns

spinqualitylabelsall models Caption for this structure Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D

About 54 residues with 6 cysteines and 2 histidines, conserved for all isoforms and species. The modification of one of the eight zinc ligands destabilize the connection to the mRNA. The domain includes :

• • • A Rubredoxin turn (Rd turn, residues 515-520), which is stabilized by hydrogen bonds between amide and sulfure.
    • β-hairpin with β1 (residues 525-527) and β2 (residues 533-535) between which there is an helical turn stabilized by hydrogen bonds.
    • An α1 helix (residues 538-545) which forms the second bridge between the two zinc-binding sites. The surface-exposed face of the helix has a potential for specific intermolecular interactions with nucleic acids or proteins.
    • A 3₁₀ helical turn (residues 550-552).
    • 2 zinc binding sites, the first one is composed of Cys515, Cys518, Cys537, Cys540 and the second is composed of Cys527, Cys532, His545 and His553.
  • RRMs patterns

Disease

This is a sample scene created with SAT to color by Group, and another to make a transparent representation of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.