6yhl | pdb_00006yhl

Crystal structure of CNFy from Yersinia pseudotuberculosis - N-terminal fragment comprising residues 1-704

spinqualitylabelsall models 6yhl, resolution 3.28Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6yhl is a 2 chain structure with sequence from "bacillus_pseudotuberkulosis"_(sic)_pfeiffer_1889 "bacillus pseudotuberkulosis" (sic) pfeiffer 1889. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: cnf ("Bacillus pseudotuberkulosis" (sic) Pfeiffer 1889) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Cytotoxic necrotizing factors (CNFs) are bacterial single-chain exotoxins that modulate cytokinetic/oncogenic and inflammatory processes through activation of host cell Rho GTPases. To achieve this, they are secreted, bind surface receptors to induce endocytosis and translocate a catalytic unit into the cytosol to intoxicate host cells. A three-dimensional structure that provides insight into the underlying mechanisms is still lacking. Here, we determined the crystal structure of full-length Yersinia pseudotuberculosis CNFY . CNFY consists of five domains (D1-D5), and by integrating structural and functional data, we demonstrate that D1-3 act as export and translocation module for the catalytic unit (D4-5) and for a fused beta-lactamase reporter protein. We further found that D4, which possesses structural similarity to ADP-ribosyl transferases, but had no equivalent catalytic activity, changed its position to interact extensively with D5 in the crystal structure of the free D4-5 fragment. This liberates D5 from a semi-blocked conformation in full-length CNFY , leading to higher deamidation activity. Finally, we identify CNF translocation modules in several uncharacterized fusion proteins, which suggests their usability as a broad-specificity protein delivery tool. Crystal structure of bacterial cytotoxic necrotizing factor CNFY reveals molecular building blocks for intoxication.,Chaoprasid P, Lukat P, Muhlen S, Heidler T, Gazdag EM, Dong S, Bi W, Ruter C, Kirchenwitz M, Steffen A, Jansch L, Stradal TEB, Dersch P, Blankenfeldt W EMBO J. 2021 Jan 7:e105202. doi: 10.15252/embj.2020105202. PMID:33410511 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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