7buu | pdb_00007buu

Eucommia ulmoides TPT3, crystal form 1

spinqualitylabelsall models 7buu, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7buu is a 2 chain structure with sequence from Eucul. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: FPS3 (EUCUL) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Some plant trans-1,4-prenyltransferases (TPTs) produce ultrahigh molecular weight trans-1,4-polyisoprene (TPI) with a molecular weight of over 1.0 million. Although plant-derived TPI has been utilized in various industries, its biosynthesis and physiological function(s) are unclear. Here, we identified three novel Eucommia ulmoides TPT isoforms-EuTPT1, 3, and 5, which synthesized TPI in vitro without other components. Crystal structure analysis of EuTPT3 revealed a dimeric architecture with a central hydrophobic tunnel. Mutation of Cys94 and Ala95 on the central hydrophobic tunnel no longer synthesizd TPI, indicating that Cys94 and Ala95 were essential for forming the dimeric architecture of ultralong-chain TPTs and TPI biosynthesis. A spatiotemporal analysis of the physiological function of TPI in E. ulmoides suggested that it is involved in seed development and maturation. Thus, our analysis provides functional and mechanistic insights into TPI biosynthesis and uncovers biological roles of TPI in plants. Structure-function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis.,Kajiura H, Yoshizawa T, Tokumoto Y, Suzuki N, Takeno S, Takeno KJ, Yamashita T, Tanaka SI, Kaneko Y, Fujiyama K, Matsumura H, Nakazawa Y Commun Biol. 2021 Feb 16;4(1):215. doi: 10.1038/s42003-021-01739-5. PMID:33594248 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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