7kws | pdb_00007kws

Cj1441 with NAD+ and UDP-glucose

spinqualitylabelsall models 7kws, resolution 2.09Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7kws is a 2 chain structure with sequence from Camje. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NAD, UPG Gene: kfiD, Cj1441c (CAMJE) Activity: UDP-glucose 6-dehydrogenase, with EC number 1.1.1.22 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Campylobacter jejuni is a pathogenic organism that can cause campylobacteriosis in children and adults. Most commonly, campylobacter infection is brought on by consumption of raw or undercooked poultry, unsanitary drinking water, or pet feces. Surrounding the C. jejuni bacterium is a coat of sugar molecules known as the capsular polysaccharide (CPS). The capsular polysaccharide can be very diverse among the different strains of C. jejuni, and this diversity is considered important for evading the host immune system. Modifications to the CPS of C. jejuni NCTC 11168 include O-methylation, phosphoramidylation, and amidation of glucuronate with either serinol or ethanolamine. The enzymes responsible for amidation of glucuronate are currently unknown. In this study, Cj1441, an enzyme expressed from the CPS biosynthetic gene cluster in C. jejuni NCTC 11168, was shown to catalyze the oxidation of UDP-alpha-d-glucose into UDP-alpha-d-glucuronic acid with NAD(+) as the cofactor. No amide products were found in an attempt to determine whether the putative thioester intermediate formed during the oxidation of UDP-glucose by Cj1441 could be captured in the presence of added amines. The three-dimensional crystal structure of Cj1441 was determined in the presence of NAD(+) and UDP-glucose bound in the active site of the enzyme (Protein Data Bank entry 7KWS). A more thorough bioinformatic analysis of the CPS gene cluster suggests that the amidation activity is localized to the t-terminal half of Cj1438, a bifunctional enzyme that is currently annotated as a sugar transferase. Functional and Structural Characterization of the UDP-Glucose Dehydrogenase Involved in Capsular Polysaccharide Biosynthesis from Campylobacter jejuni.,Riegert AS, Raushel FM Biochemistry. 2021 Mar 9;60(9):725-734. doi: 10.1021/acs.biochem.0c00953. Epub, 2021 Feb 23. PMID:33621065 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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