6kj5 | pdb_00006kj5

Crystal structure of 10-Hydroxygeraniol Dehydrogenase apo form from Cantharanthus roseus

spinqualitylabelsall models 6kj5, resolution 3.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6kj5 is a 1 chain structure with sequence from Catro. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ZN Gene: 10HGO (CATRO) Activity: 8-hydroxygeraniol dehydrogenase, with EC number 1.1.1.324 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Conversion of 10-hydroxygeraniol to 10-oxogeranial is a crucial step in iridoid biosynthesis. This reaction is catalyzed by a zinc-dependent alcohol dehydrogenase, 10-hydroxygeraniol dehydrogenase, belonging to the family of medium-chain dehydrogenase/reductase (MDR). Here, we report the crystal structures of a novel 10-hydroxygeraniol dehydrogenase from Catharanthus roseus in its apo and nicotinamide adenine dinucleotide phosphate (NADP(+) ) bound forms. Structural analysis and docking studies reveal how subtle conformational differences of loops L1, L2, L3, and helix alpha9' at the orifice of the catalytic site confer differential activity of the enzyme toward various substrates, by modulating the binding pocket shape and volume. The present study, first of its kind, provides insights into the structural basis of substrate specificity of MDRs specific to linear substrates. Furthermore, comparison of apo and NADP(+) bound structures suggests that the enzyme adopts open and closed states to facilitate cofactor binding. Structural studies on 10-hydroxygeraniol dehydrogenase: A novel linear substrate-specific dehydrogenase from Catharanthus roseus.,Sandholu AS, Mujawar SP, Ramakrishnan K, Thulasiram HV, Kulkarni K Proteins. 2020 Sep;88(9):1197-1206. doi: 10.1002/prot.25891. Epub 2020 Mar 26. PMID:32181958 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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