7bbi | pdb_00007bbi

Joint X-ray/neutron room temperature structure of H/D-exchanged PLL lectin

spinqualitylabelsall models 7bbi, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7bbi is a 1 chain structure with sequence from Ccm 7076. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: CKY10_20885 (CCM 7076) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Carbohydrate-binding proteins from pathogenic bacteria and fungi have been shown to be implicated in various pathological processes, where they interact with glycans present on the surface of the host cells. These interactions are part of the initial processes of infection of the host and are very important to study at the atomic level. Here, we report the room temperature neutron structures of PLL lectin from Photorhabdus laumondii in its apo form and in complex with deuterated L-fucose, which is, to our knowledge, the first neutron structure of a carbohydrate-binding protein in complex with a fully deuterated carbohydrate ligand. A detailed structural analysis of the lectin-carbohydrate interactions provides information on the hydrogen bond network, the role of water molecules, and the extent of the CH-pi stacking interactions between fucose and the aromatic amino acids in the binding site. Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions.,Gajdos L, Blakeley MP, Kumar A, Wimmerova M, Haertlein M, Forsyth VT, Imberty A, Devos JM Structure. 2021 Mar 24. pii: S0969-2126(21)00076-9. doi:, 10.1016/j.str.2021.03.003. PMID:33765407 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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