7kog | pdb_00007kog

Lethocerus Myosin II complete coiled-coil domain resolved in its native environment

spinqualitylabelsall models 7kog, resolution 4.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7kog is a 2 chain structure with sequence from Lethocerus indicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The atomic structure of the complete myosin tail within thick filaments isolated from Lethocerus indicus flight muscle is described and compared to crystal structures of recombinant, human cardiac myosin tail segments. Overall, the agreement is good with three exceptions: the proximal S2, in which the filament has heads attached but the crystal structure doesn't, and skip regions 2 and 4. At the head-tail junction, the tail alpha-helices are asymmetrically structured encompassing well-defined unfolding of 12 residues for one myosin tail, approximately 4 residues of the other, and different degrees of alpha-helix unwinding for both tail alpha-helices, thereby providing an atomic resolution description of coiled-coil "uncoiling" at the head-tail junction. Asymmetry is observed in the nonhelical C termini; one C-terminal segment is intercalated between ribbons of myosin tails, the other apparently terminating at Skip 4 of another myosin tail. Between skip residues, crystal and filament structures agree well. Skips 1 and 3 also agree well and show the expected alpha-helix unwinding and coiled-coil untwisting in response to skip residue insertion. Skips 2 and 4 are different. Skip 2 is accommodated in an unusual manner through an increase in alpha-helix radius and corresponding reduction in rise/residue. Skip 4 remains helical in one chain, with the other chain unfolded, apparently influenced by the acidic myosin C terminus. The atomic model may shed some light on thick filament mechanosensing and is a step in understanding the complex roles that thick filaments of all species undergo during muscle contraction. The myosin II coiled-coil domain atomic structure in its native environment.,Rahmani H, Ma W, Hu Z, Daneshparvar N, Taylor DW, McCammon JA, Irving TC, Edwards RJ, Taylor KA Proc Natl Acad Sci U S A. 2021 Apr 6;118(14). pii: 2024151118. doi:, 10.1073/pnas.2024151118. PMID:33782130 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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