6k6n | pdb_00006k6n

Crystal structure of SIVmac239 Nef protein

spinqualitylabelsall models 6k6n, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6k6n is a 1 chain structure with sequence from Civ. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: nef (CIV) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The human immunodeficiency virus (HIV) accessory protein Nef plays a major role in establishing and maintaining infection, particularly through immune evasion. Many HIV-2-infected people experience long-term viral control and survival, resembling HIV-1 elite control. HIV-2 Nef has overlapping but also distinct functions from HIV-1 Nef. Here we report the crystal structure of HIV-2 Nef core. The di-leucine sorting motif forms a helix bound to neighboring molecules, and moreover, isothermal titration calorimetry demonstrated that the CD3 endocytosis motif can directly bind to HIV-2 Nef, ensuring AP-2-mediated endocytosis for CD3. The highly conserved C-terminal region forms a alpha-helix, absent from HIV-1. We further determined the structure of simian immunodeficiency virus (SIV) Nef harboring this region, demonstrating similar C-terminal alpha-helix, which may contribute to AP-1 binding for MHC-I downregulation. These results provide insights into the distinct pathogenesis of HIV-2 infection. Structure of HIV-2 Nef Reveals Features Distinct from HIV-1 Involved in Immune Regulation.,Hirao K, Andrews S, Kuroki K, Kusaka H, Tadokoro T, Kita S, Ose T, Rowland-Jones SL, Maenaka K iScience. 2020 Jan 24;23(1):100758. doi: 10.1016/j.isci.2019.100758. Epub 2019, Dec 9. PMID:31927483 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Protein Nef

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