7brs | pdb_00007brs

E.coli beta-galactosidase (E537Q) in complex with fluorescent probe KSA02

spinqualitylabelsall models 7brs, resolution 2.67Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7brs is a 4 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: DMS, F4X, GOL, MG, NA Gene: lacZ, b0344, JW0335 (ECOLI) Activity: Beta-galactosidase, with EC number 3.2.1.23 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The tracking of cellular senescence usually depends on the detection of senescence-associated beta-galactosidase (SA-beta-gal). Previous probes for SA-beta-gal with this purpose only cover a single dimension: the accumulation of this enzyme in lysosomes. However, this is insufficient to determine the destiny of senescence because endogenous beta-gal enriched in lysosomes is not only related to senescence, but also to some other physiological processes. To address this issue, we introduce our fluorescent probes including a second dimension: lysosomal pH, since de-acidification is a unique feature of the lysosomes in senescent cells. With this novel design, our probes achieved excellent discrimination of SA-beta-gal from cancer-associated beta-gal, which enables them to track cellular senescence as well as tissue aging more precisely. Our crystal structures of a model enzyme E. coli beta-gal mutant (E537Q) complexed with each probe further revealed the structural basis for probe recognition. Two-Dimensional Design Strategy to Construct Smart Fluorescent Probes for the Precise Tracking of Senescence.,Guo Y, Gao Y, Hu Y, Liu Q, Li X, Li X, Kim CY, James TD, Li J, Chen X Angew Chem Int Ed Engl. 2021 Feb 24. doi: 10.1002/anie.202101278. PMID:33624914 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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