Structural highlights
Publication Abstract from PubMedThere is a common interest for studying xeno-nucleic acid systems in the fields of synthetic biology and the origin of life, in particular, those with an engineered backbone and possessing novel properties. Along this line, we have investigated xylonucleic acid (XyloNA) containing a potentially prebiotic xylose sugar (a 3'-epimer of ribose) in its backbone. Herein, we report for the first time the synthesis of four XyloNA nucleotide building blocks and the assembly of XyloNA oligonucleotides containing all the natural nucleobases. A detailed investigation of pairing and structural properties of XyloNAs in comparison to DNA/RNA has been performed by thermal UV-melting, CD, and solution state NMR spectroscopic studies. XyloNA has been shown to be an orthogonal self-pairing system which adopts a slightly right-handed extended helical geometry. Our study on one hand, provides understanding for superior structure-function (-pairing) properties of DNA/RNA over XyloNA for selection as an informational polymer in the prebiotic context, while on the other hand, finds potential of XyloNA as an orthogonal genetic system for application in synthetic biology. Xylonucleic acid: synthesis, structure, and orthogonal pairing properties.,Maiti M, Maiti M, Knies C, Dumbre S, Lescrinier E, Rosemeyer H, Ceulemans A, Herdewijn P Nucleic Acids Res. 2015 Jul 14. pii: gkv719. PMID:26175047[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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