2v5u | pdb_00002v5u

I92A FLAVODOXIN FROM ANABAENA

spinqualitylabelsall models 2v5u, resolution 1.99Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2v5u is a 2 chain structure with sequence from Anabaena pcc7119. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FMN Related: 1dx9, 1ftg, 1obo, 1obv, 1qhe, 2v5v Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [FLAV_NOSSO] Low-potential electron donor to a number of redox enzymes. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Contribution of three regions (phosphate-binding, 50's and 90's loops) of Anabaena apoflavodoxin to FMN binding and reduction potential was studied. Thr12 and Glu16 did not influence FMN redox properties, but Thr12 played a role in FMN binding. Replacement of Trp57 with Glu, Lys or Arg moderately shifted E(ox/sq) and E(sq/hq) and altered the energetic of the FMN redox states binding profile. Our data indicate that the side chain of position 57 does not modulate E(ox/sq) by aromatic stacking or solvent exclusion, but rather by influencing the relative strength of the H-bond between the N(5) of the flavin and the Asn58-Ile59 bond. A correlation was observed between the isoalloxazine increase in solvent accessibility and less negative E(sq/hq). Moreover, E(sq/hq) became less negative as positively charged residues were added near to the isoalloxazine. Ile59 and Ile92 were simultaneously mutated to Ala or Glu. These mutations impaired FMN binding, while shifting E(sq/hq) to less negative values and E(ox/sq) to more negative. These effects are discussed on the bases of the X-ray structures of some of the Fld mutants, suggesting that in Anabaena Fld the structural control of both electron transfer steps is much more subtle than in other Flds. Tuning of the FMN binding and oxido-reduction properties by neighboring side chains in Anabaena flavodoxin.,Frago S, Goni G, Herguedas B, Peregrina JR, Serrano A, Perez-Dorado I, Molina R, Gomez-Moreno C, Hermoso JA, Martinez-Julvez M, Mayhew SG, Medina M Arch Biochem Biophys. 2007 Nov 15;467(2):206-17. Epub 2007 Aug 29. PMID:17904516 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Flavodoxin 3D structures

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