1tcs | pdb_00001tcs

CRYSTAL STRUCTURE OF TRICHOSANTHIN-NADPH COMPLEX AT 1.7 ANGSTROMS RESOLUTION REVEALS ACTIVE-SITE ARCHITECTURE

spinqualitylabelsall models 1tcs, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1tcs is a 1 chain structure with sequence from Chinese cucumber. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NDP Activity: rRNA N-glycosylase, with EC number 3.2.2.22 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [RIPT_TRIKI] Inactivates eukaryotic 60S ribosomal subunits. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed We describe here the crystal structure of the trichosanthin-NADPH complex determined at a resolution of 1.7 A. The adenine base stacks between Tyr 70 and Tyr 111. Arg 163, Glu 160 and Tyr 70 form hydrogen bonds to N(3), O(3') and, through a water molecule, to N(9) of adenosine, respectively. This is the first high resolution structure of a complex between a ribosome-inactivating protein and a substrate analogue, in which the electron density of the N-glycosidic bond is well defined and the preassociated water, thought to be responsible for hydrolyzing the N-C bond, is also explicitly elucidated. Crystal structure of trichosanthin-NADPH complex at 1.7 A resolution reveals active-site architecture.,Xiong JP, Xia ZX, Wang Y Nat Struct Biol. 1994 Oct;1(10):695-700. PMID:7634073 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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