7app | pdb_00007app

Structure of Lipase TL from capillary grown crystal in the presence of agarose

spinqualitylabelsall models 7app, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7app is a 2 chain structure with sequence from Cbs 632.91. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FMT, NA, NAG Related: 7apn Gene: LIP (CBS 632.91) Activity: Triacylglycerol lipase, with EC number 3.1.1.3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The autoimmobilization of enzymes via cross-linked enzyme crystals (CLECs) has regained interest in recent years, boosted by the extensive knowledge gained in protein crystallization, the decrease of cost and laboriousness of the process, and the development of potential applications. In this work, we present the crystallization and preparative-scale production of reinforced cross-linked lipase crystals (RCLLCs) using a commercial detergent additive as a raw material. Bulk crystallization was carried out in 500 mL of agarose media using the batch technique. Agarose facilitates the homogeneous production of crystals, their cross-linking treatment, and their extraction. RCLLCs were active in an aqueous solution and in hexane, as shown by the hydrolysis of p-nitrophenol butyrate and alpha-methylbenzyl acetate, respectively. RCLLCs presented both high thermal and robust operational stability, allowing the preparation of a packed-bed chromatographic column to work in a continuous flow. Finally, we determined the three-dimensional (3D) models of this commercial lipase crystallized with and without phosphate at 2.0 and 1.7 A resolutions, respectively. Production of Cross-Linked Lipase Crystals at a Preparative Scale.,Fernandez-Penas R, Verdugo-Escamilla C, Martinez-Rodriguez S, Gavira JA Cryst Growth Des. 2021 Mar 3;21(3):1698-1707. doi: 10.1021/acs.cgd.0c01608. Epub , 2021 Feb 17. PMID:34602865 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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