1ig9 | pdb_00001ig9

Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase

spinqualitylabelsall models 1ig9, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1ig9 is a 3 chain structure with sequence from Bpr69. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, TTP NonStd Res: DOC Related: 1waj, 1clq Gene: gp43 (BPR69) Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [DPOL_BPR69] This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed We describe the 2.6 A resolution crystal structure of RB69 DNA polymerase with primer-template DNA and dTTP, capturing the step just before primer extension. This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation of the fingers domain relative to the apo-protein structure, similar to the fingers movement in pol I family polymerases. Minor groove interactions near the primer 3' terminus suggest a common fidelity mechanism for pol I and pol alpha family polymerases. The duplex product DNA orientation differs by 40 degrees between the polymerizing mode and editing mode structures. The role of the thumb in this DNA motion provides a model for editing in the pol alpha family. Structure of the replicating complex of a pol alpha family DNA polymerase.,Franklin MC, Wang J, Steitz TA Cell. 2001 Jun 1;105(5):657-67. PMID:11389835 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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