2a6u | pdb_00002a6u

pH evolution of tetragonal HEWL at 4 degrees Celcius.

spinqualitylabelsall models 2a6u Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2a6u is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Activity: Lysozyme, with EC number 3.2.1.17 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed 44 samples of tetragonal hen egg-white lysozyme (HEWL) were obtained as a series of polycrystalline precipitates at 277 K and room temperature in the pH range between 6.56 and 3.33. The precipitates were investigated by the collection of high-resolution powder X-ray diffraction data at 295 K, which reveal the tetragonal or orthorhombic forms of lysozyme depending on the temperature and pH of crystallization. The use of a new robotic sample changer greatly facilitated these measurements. LeBail analyses of the powder patterns display a characteristic behaviour for the pH dependence of the tetragonal unit-cell parameters of HEWL crystallized at both temperatures. More detailed analysis shows that molecular replacement can give a suitable starting point for structural refinements, illustrating that powder data can be sufficient for this approach. Pawley or Rietveld refinements that fit a single model to four data sets simultaneously from four samples crystallized at pH values across the range studied benefit from improved powder data quality via the anisotropic changes in the unit cell. The Rietveld analysis gave an average structural model with excellent goodness of fit and stereochemistry. High-throughput phase-diagram mapping via powder diffraction: a case study of HEWL versus pH.,Basso S, Fitch AN, Fox GC, Margiolaki I, Wright JP Acta Crystallogr D Biol Crystallogr. 2005 Dec;61(Pt 12):1612-25. Epub 2005, Nov 19. PMID:16301795 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Lysozyme 3D structures

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