6pl0 | pdb_00006pl0

Crystal structure of the dark-adapted full-length bacteriophytochrome XccBphP from Xanthomonas campestris in the Pr state bound to BV chromophore

Structural highlights

6pl0 is a 2 chain structure with sequence from Xanc8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	BLA
Related:	5akp
Gene:	bphP, XC_4241 (XANC8)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[BPHY_XANC8] Photoreceptor which exists in two forms that are reversibly interconvertible by light: far-red light (733 nm) converts protein to the red-absorbing (Pr) form, while red light (630 nm) partly converts the protein to the far-red-absorbing (Pfr) form (PubMed:27107635). Regulates virulence of X.campestris pv. campestris on its host plants, perhaps by fine-tuning expression to ambient light levels and/or spatial cues (PubMed:27621284). The Pr form may sense light and partially inhibit virulence; in the dark (Pfr form) biofilm and xanathan production rise and bacteria are more virulent (PubMed:27621284). Strains overexpressing this protein have significantly decreased amounts of extracellular beta-1,4-endoglucanase, produce less xanthin and have decreased transcription of genes involved in virulence such as endoglucanases, type 2 secretion systems, xanthan production and flagellar-dependent motility (PubMed:27621284).^[1] ^[2] ^[3]

Publication Abstract from PubMed

[Figure: see text].

Structural basis for the Pr-Pfr long-range signaling mechanism of a full-length bacterial phytochrome at the atomic level.,Otero LH, Foscaldi S, Antelo GT, Rosano GL, Sirigu S, Klinke S, Defelipe LA, Sanchez-Lamas M, Battocchio G, Conforte V, Vojnov AA, Chavas LMG, Goldbaum FA, Mroginski MA, Rinaldi J, Bonomi HR Sci Adv. 2021 Nov 26;7(48):eabh1097. doi: 10.1126/sciadv.abh1097. Epub 2021 Nov, 24. PMID:34818032^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Otero LH, Klinke S, Rinaldi J, Velazquez-Escobar F, Mroginski MA, Fernandez Lopez M, Malamud F, Vojnov AA, Hildebrandt P, Goldbaum FA, Bonomi HR. Structure of the Full-Length Bacteriophytochrome from the Plant Pathogen Xanthomonas campestris Provides Clues to its Long-Range Signaling Mechanism. J Mol Biol. 2016 Apr 20. pii: S0022-2836(16)30071-7. doi:, 10.1016/j.jmb.2016.04.012. PMID:27107635 doi:https://dx.doi.org/10.1016/j.jmb.2016.04.012
↑ Bonomi HR, Toum L, Sycz G, Sieira R, Toscani AM, Gudesblat GE, Leskow FC, Goldbaum FA, Vojnov AA, Malamud F. Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor. EMBO Rep. 2016 Nov;17(11):1565-1577. doi: 10.15252/embr.201541691. Epub 2016 Sep , 12. PMID:27621284 doi:https://dx.doi.org/10.15252/embr.201541691
↑ Bonomi HR, Toum L, Sycz G, Sieira R, Toscani AM, Gudesblat GE, Leskow FC, Goldbaum FA, Vojnov AA, Malamud F. Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor. EMBO Rep. 2016 Nov;17(11):1565-1577. doi: 10.15252/embr.201541691. Epub 2016 Sep , 12. PMID:27621284 doi:https://dx.doi.org/10.15252/embr.201541691
↑ Otero LH, Foscaldi S, Antelo GT, Rosano GL, Sirigu S, Klinke S, Defelipe LA, Sanchez-Lamas M, Battocchio G, Conforte V, Vojnov AA, Chavas LMG, Goldbaum FA, Mroginski MA, Rinaldi J, Bonomi HR. Structural basis for the Pr-Pfr long-range signaling mechanism of a full-length bacterial phytochrome at the atomic level. Sci Adv. 2021 Nov 26;7(48):eabh1097. doi: 10.1126/sciadv.abh1097. Epub 2021 Nov, 24. PMID:34818032 doi:https://dx.doi.org/10.1126/sciadv.abh1097

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Otero LH, Klinke S, Rinaldi J, Velazquez-Escobar F, Mroginski MA, Fernandez Lopez M, Malamud F, Vojnov AA, Hildebrandt P, Goldbaum FA, Bonomi HR. Structure of the Full-Length Bacteriophytochrome from the Plant Pathogen Xanthomonas campestris Provides Clues to its Long-Range Signaling Mechanism. J Mol Biol. 2016 Apr 20. pii: S0022-2836(16)30071-7. doi:, 10.1016/j.jmb.2016.04.012. PMID:27107635 doi:https://dx.doi.org/10.1016/j.jmb.2016.04.012

[2] Bonomi HR, Toum L, Sycz G, Sieira R, Toscani AM, Gudesblat GE, Leskow FC, Goldbaum FA, Vojnov AA, Malamud F. Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor. EMBO Rep. 2016 Nov;17(11):1565-1577. doi: 10.15252/embr.201541691. Epub 2016 Sep , 12. PMID:27621284 doi:https://dx.doi.org/10.15252/embr.201541691

[3] Bonomi HR, Toum L, Sycz G, Sieira R, Toscani AM, Gudesblat GE, Leskow FC, Goldbaum FA, Vojnov AA, Malamud F. Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor. EMBO Rep. 2016 Nov;17(11):1565-1577. doi: 10.15252/embr.201541691. Epub 2016 Sep , 12. PMID:27621284 doi:https://dx.doi.org/10.15252/embr.201541691

[4] Otero LH, Foscaldi S, Antelo GT, Rosano GL, Sirigu S, Klinke S, Defelipe LA, Sanchez-Lamas M, Battocchio G, Conforte V, Vojnov AA, Chavas LMG, Goldbaum FA, Mroginski MA, Rinaldi J, Bonomi HR. Structural basis for the Pr-Pfr long-range signaling mechanism of a full-length bacterial phytochrome at the atomic level. Sci Adv. 2021 Nov 26;7(48):eabh1097. doi: 10.1126/sciadv.abh1097. Epub 2021 Nov, 24. PMID:34818032 doi:https://dx.doi.org/10.1126/sciadv.abh1097

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6pl0 | pdb_00006pl0

Crystal structure of the dark-adapted full-length bacteriophytochrome XccBphP from Xanthomonas campestris in the Pr state bound to BV chromophore

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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