Structural highlights
Publication Abstract from PubMedNAD metabolism is essential for all forms of life. Compartmental regulation of NAD(+) consumption, especially between the nucleus and the mitochondria, is required for energy homeostasis. However, how compartmental regulation evolved remains unclear. In the present study, we investigated the evolution of the macrodomain-containing histone variant macroH2A1.1, an integral chromatin component that limits nuclear NAD(+) consumption by inhibiting poly(ADP-ribose) polymerase 1 in vertebrate cells. We found that macroH2A originated in premetazoan protists. The crystal structure of the macroH2A macrodomain from the protist Capsaspora owczarzaki allowed us to identify highly conserved principles of ligand binding and pinpoint key residue substitutions, selected for during the evolution of the vertebrate stem lineage. Metabolic characterization of the Capsaspora lifecycle suggested that the metabolic function of macroH2A was associated with nonproliferative stages. Taken together, we provide insight into the evolution of a chromatin element involved in compartmental NAD regulation, relevant for understanding its metabolism and potential therapeutic applications. Evolution of a histone variant involved in compartmental regulation of NAD metabolism.,Guberovic I, Hurtado-Bages S, Rivera-Casas C, Knobloch G, Malinverni R, Valero V, Leger MM, Garcia J, Basquin J, Gomez de Cedron M, Frigole-Vivas M, Cheema MS, Perez A, Ausio J, Ramirez de Molina A, Salvatella X, Ruiz-Trillo I, Eirin-Lopez JM, Ladurner AG, Buschbeck M Nat Struct Mol Biol. 2021 Dec;28(12):1009-1019. doi: 10.1038/s41594-021-00692-5. , Epub 2021 Dec 9. PMID:34887560[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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