Protein Hfq

spinqualitylabelsall models Structure of E. coli hexameric Hfq complex with poly(A) RNA and poly(U) RNA (PDB code 4ht9) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Protein Hfq (Hfq) (Host Factor for Qβ) or RNA-binding protein Hfq is stimulating base-pairing between sRNA and target mRNA by binding both RNAs via three RNA-binding surfaces. Hfq is found in enteric bacteria. Hfq binds RNA via poly(A-R-N) triplets where A is adenosine, R is purine nucleotide and N is any nucleotide. Relevance Since Hfq is required for gene regulation and infectivity of some Gram-negative bacteria its mutations can eliminate infectivity of Lyme disease caused by the bacteria Borellia burgdorferi, for example. Structural highlights The biological assembly of Protein Hfq from E. coli is homohexamer (PDB code 4ht9). The 3D structure of the complex between Hfq and poly(A) RNA and poly(U) RNA shows the poly(A) binding at the distal face of Hfq and poly(U) binding at its proximal face. For the poly(A) A-R-N binding site the A site shows hydrogen bonding to Gln and Lys. The R site shows stacking interactions as well as hydrogen bonding. The N site does not show any interaction. The poly(U) binding site of Hfq is a classical uridine binding pocket with both stacking and hydrogen bonding interactions.

3D Structures of protein Hfq

Updated on 03-February-2022

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• Protein Hfq
  • 4ht9, 2y90, 2yht, 3qhs, 4rcb, 4rcc, 6bdg – EcHfq – Escherichia coli
    
  • 4jli, 4jri, 4jrk, 4juv – EcHfq (mutant)
    
  • 1u1s, 1u1t, 4j6y, 6xyj – PaHfq – Pseudomonas aeruginosa
    
  • 3inz, 3m4g, 4mml, 4mmk, 5i21 – PaHfq (mutant)
    
  • 2ylb – StHfq – Salmonella typhimurium
    
  • 6gwk – Hfq – Caulobacter crescentus
    
  • 4nl2 – LmHfq – Listeria monocytogenes
    
  • 4noy – LmHfq (mutant)
    
  • 5szd – AaHfq – Aquifex aeolicus
    
  • 3sb2 – Hfq - Herbaspirillum seropedicae
    
  • 1kq1 – SaHfq – Staphylococcus aureus
    
  • 2qtx, 4x9c – MjHfq – Methanococcus jannaschii
    
  • 3hfn – Hfq - Anabaena
    
• Protein Hfq complex with nucleotide
  • 3res – EcHfq + ADP
    
  • 3qo3 – EcHfq + ATP
    
  • 4pno – EcHfq + UMP
    
  • 4j6x – PaHfq + UTP
    
  • 3qui – PaHfq + ADPNP
    
  • 4j5y – PaHfq + ATP
    
  • 4j6w – PaHfq + CTP
    
  • 2ylc – StHfq + UMP
    
  • 5dy9 – MjHfq (mutant) + AMP
    
  • 4x9d – MjHfq + UMP
    
• Protein Hfq other complexes
  • 6qlb – EcHfq + calpain
    
  • 3vu3 – EcHfq + catalase
    
  • 3gib, 4ht8 – EcHfq + poly(A)
    
  • 4ht9, 5new – EcHfq + poly(A) + poly(U)
    
  • 3rer – EcHfq + poly(U) + ADP
    
  • 4qvc, 4qvd – EcHfq + RNA
    
  • 5uk7 – EcHfq + DNA
    
  • 7ogm – EcHfq + PNPase + RNA – Cryo EM
    
  • 3ahu, 3hsb – Hfq + RNA – Bacillus subtilis
    
  • 6o1k, 6o1l, 6o1m – PaHfq + Crc + RNA – Cryo EM
    
  • 4v2s – StHfq + sRNA
    
  • 4y91 – Hfq + poly(U) – Thermotoga maritima
    
  • 4nl3 – LmHfq + poly(U)
    
  • 5sze – AaHfq + poly(U)
    
  • 1kq2 – SaHfq + RNA
    

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